To elucidate the mechanisms behind the robust functioning of soil microbes within soil structure, we will examine the relationship between physicochemical properties and soil microbes involved in N cycle. This is achieved by (i) identifying the key factors controlling soil aggregation and microbial functioning within aggregates, and (ii) building artificial aggregates that hold key soil functions.

Soil microorganisms driving carbon (C) and nitrogen (N) cycle are largely present in soil aggregate – the porous structure consisting of soil mineral particles and organic matter. The variation in the physical and chemical features of these microhabitats likely exert strong control on the difference in soil microbiome and in the dynamics of greenhouse gas emission. The current project aims to understand how soil structure control the stability and functioning of soil microbiome using three general approaches: (1) non-destructive characterization of physicochemical and microbial features of soil aggregates, (2) step-wise destruction and physical fractionation of soil aggregates, and (3) artificial soil aggregate synthesis.

Soil microorganisms driving carbon (C) and nitrogen (N) cycle are largely present in soil aggregate – the porous structure consisting of soil mineral particles and organic matter. The variation in the physical and chemical features of these microhabitats likely exert strong control on the difference in soil microbiome and in the dynamics of greenhouse gas emission. The current project aims to understand how soil structure control the stability and functioning of soil microbiome using three general approaches: (1) non-destructive characterization of physicochemical and microbial features of soil aggregates, (2) step-wise destruction and physical fractionation of soil aggregates, and (3) artificial soil aggregate synthesis.

2021年度
Rota Wagai
National Agriculture and Food Research Organization, NARO
Principal Researcher
⦾ Field of research Soil biogeochemistry
⦾ Key Words Aggregate structure, organo-mineral interaction, C & N daynamics
Satoshi Mitsunobu
Ehime University
Associate Professor
⦾ Field of research Soil Chemistry, Geochemistry
⦾ Key Words Soil, Chemical speciation, Synchrotron
Kazumichi Fujii
Forestry and Forest Products Research Institute
Senior researcher
⦾ Field of research Soil Science
⦾ Key Words Soil organic matter
Noriko Yamaguchi
National Agriculture and Food Research Organization, NARO
Assistant Group Leader
⦾ Field of research Soil chemistry
⦾ Key Words spectroscopy
Aomi Suda
National Agriculture and Food Research Organization, NARO
Senior Scientist
⦾ Field of research Inorganic Soil Chemistry
⦾ Key Words Soil, redox reaction, trace elements
Jinsen ZHENG
Forestry and Forest Products Research Institute
Postdoctoral Researcher
⦾ Field of research Soil biogeochemistry
⦾ Key Words nitrogen biogeochemistry; greenhouse gas emission; soil fertility management
Hiroaki Shimada
National Agriculture and Food Research Organization, NARO
Postdoctoral Fellow
⦾ Field of research Soil Science
⦾ Key Words Organo-metal association, Soil genesis and classification
Ryo Shingubara
National Agriculture and Food Research Organization, NARO
Postdoctoral Fellow
⦾ Field of research Biogeochemistry, Stable isotope geochemistry, Biogeoscience
⦾ Key Words Stable isotope ratio, Greenhouse gas, Methane, Arctic, Eastern Siberia, Wetland, Volcanic gas
Koji Ito
National Agriculture and Food Research Organization, NARO
Postdoctoral Fellow
⦾ Field of research Soil bacteriology
⦾ Key Words Persistent organic pollutant, biodegradation, dehalogenase gene
Emi Matsumura
National Agriculture and Food Research Organization, NARO
Postdoctoral Fellow
⦾ Field of research Forest Microbial Ecology, Forest Pathology
⦾ Key Words Community ecology、Endophyte
To reveal soil microbial functions and utilize their full potential, advanced technologies play key roles. In this section, we will focus on new technologies such as single-cell genomics, long-read sequencing, bioinformatics, and network analysis.

Our group develops advanced technologies to clarify the diversity of soil microbial functions and the mechanism of soil microbiome stabilization, and to maximize their potential. In addition, we use these technologies to elucidate the entire picture of soil microorganisms.
Specifically, we are engaged in research on the following technologies:
- Long DNA sequencing
- Microdroplet
- Single-cell sequencing
- Bioinformatics
- Network analysis

Our group develops advanced technologies to clarify the diversity of soil microbial functions and the mechanism of soil microbiome stabilization, and to maximize their potential. In addition, we use these technologies to elucidate the entire picture of soil microorganisms.
Specifically, we are engaged in research on the following technologies:
- Long DNA sequencing
- Microdroplet
- Single-cell sequencing
- Bioinformatics
- Network analysis
2021年度
Wataru Iwasaki
The University of Tokyo
Professor
⦾ Field of research Bioinformatics
⦾ Key Words Genome Evolution, Metagenomics, Function Unknown Sequences
⦾ Field of research Plant–microbe interaction
⦾ Key Words Plant–microbe interaction
Hirokazu Toju
Kyoto University
Associate Professor
⦾ Field of research Ecology, Evolutionary Biology
⦾ Key Words Species Interactions, Biodiversity, Microbiology
Megumi Narukawa
RIKEN BioResource Research Center
Research & Development Scientist
⦾ Field of research Plant-Microbe Symbiosis
⦾ Key Words Soil microorganisms
Soichiro Tsuda
bitBiome Inc.
Executive Officer R&D
⦾ Field of research Microbial single-cell genome sequencing
⦾ Key Words Synthetic biology, bacteriophage, antimicrobial resistance
Sachiko Masuda
RIKEN Center for Sustainable Resource Science
Research Scientist
⦾ Field of research Long-read metagenome of plant microbiome
⦾ Key Words Plant-microbiome, metagenome
Mizue Anda
The University of Tokyo
Project Researcher
⦾ Field of research Microbial Genomics, Microbial Ecology
⦾ Key Words
Eric Chun Hung Chen
The University of Tokyo
Project Researcher
⦾ Field of research Bioinformatics, Comparative Genomics
⦾ Key Words
Tomohisa Shimasaki
RIKEN
BioResource center
Postdoctoral Researcher
⦾ Field of research Plant-microbe Interactions
⦾ Key Words Metagenomics
⦾ Field of research Symbiotic microbiology
⦾ Key Words Earth worm, microbial symbiont